streptavidin coverslips (PolyAn GmbH)
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Streptavidin Coverslips, supplied by PolyAn GmbH, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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1) Product Images from "Porcine sperm bind to an oviduct glycan coupled to glass surfaces as a model of sperm interaction with the oviduct"
Article Title: Porcine sperm bind to an oviduct glycan coupled to glass surfaces as a model of sperm interaction with the oviduct
Journal: Scientific Reports
doi: 10.1038/s41598-025-88986-2
Figure Legend Snippet: Schematic representation of the two in vitro systems developed to model the sperm-oviduct interaction. ( a ) 3-D functionalized streptavidin coverslip: the coverslips are placed on a petri dish, medium droplets containing sulfated Le X (suLe X ) are placed on the streptavidin regions of the coverslip that are surrounded by hydrophobic surface, and the whole system is covered with mineral oil. ( b ) Polyethyleneglycol (PEG)-biotin slides: the slides are coated with streptavidin, a hybridization chamber is placed on the slide, and the chambers are filled with medium containing suLe X . ( c ) The streptavidin coating in both glass surfaces allows biotinylated suLe X to be tightly immobilized to the surface. Once the sperm are added and incubated in the system, membrane receptors on the head recognize and attach to suLe X bound to the glass.
Techniques Used: In Vitro, Hybridization, Incubation, Membrane
Figure Legend Snippet: Schematic representation of IVF using sperm bound to glycans pre-fertilization (glycan-IVF). Functionalized streptavidin-coverslips were coated with suLe X in droplets of medium. Sperm were added to the droplets and incubated to allow them to bind suLe X . The medium in the droplet was replaced to remove free sperm. Mature cumulus-oocyte complexes (COCs) were added to the droplet and co-cultured with bound sperm. COCs induce the release of some of the sperm enabling them to fertilize the oocytes.
Techniques Used: Incubation, Cell Culture
Related Articles
In Vitro:Article Title: Porcine sperm bind to an oviduct glycan coupled to glass surfaces as a model of sperm interaction with the oviduct. Article Snippet: Two functionalized glass surfaces were used to immobilize oviduct glycans and model the oviduct reservoir (Fig. 1): Article Title: Porcine sperm bind to an oviduct glycan coupled to glass surfaces as a model of sperm interaction with the oviduct Article Snippet: Two functionalized glass surfaces were used to immobilize oviduct glycans and model the oviduct reservoir (Fig. ): Hybridization:Article Title: Porcine sperm bind to an oviduct glycan coupled to glass surfaces as a model of sperm interaction with the oviduct. Article Snippet: Two functionalized glass surfaces were used to immobilize oviduct glycans and model the oviduct reservoir (Fig. 1): Article Title: Porcine sperm bind to an oviduct glycan coupled to glass surfaces as a model of sperm interaction with the oviduct Article Snippet: Two functionalized glass surfaces were used to immobilize oviduct glycans and model the oviduct reservoir (Fig. ): Incubation:Article Title: Porcine sperm bind to an oviduct glycan coupled to glass surfaces as a model of sperm interaction with the oviduct. Article Snippet: Two functionalized glass surfaces were used to immobilize oviduct glycans and model the oviduct reservoir (Fig. 1): Article Title: Porcine sperm bind to an oviduct glycan coupled to glass surfaces as a model of sperm interaction with the oviduct Article Snippet: Two functionalized glass surfaces were used to immobilize oviduct glycans and model the oviduct reservoir (Fig. ): Membrane:Article Title: Porcine sperm bind to an oviduct glycan coupled to glass surfaces as a model of sperm interaction with the oviduct. Article Snippet: Two functionalized glass surfaces were used to immobilize oviduct glycans and model the oviduct reservoir (Fig. 1): Article Title: Porcine sperm bind to an oviduct glycan coupled to glass surfaces as a model of sperm interaction with the oviduct Article Snippet: Two functionalized glass surfaces were used to immobilize oviduct glycans and model the oviduct reservoir (Fig. ): Cell Culture:Article Title: Porcine sperm bind to an oviduct glycan coupled to glass surfaces as a model of sperm interaction with the oviduct. Article Snippet: Two functionalized glass surfaces were used to immobilize oviduct glycans and model the oviduct reservoir (Fig. 1): Article Title: Porcine sperm bind to an oviduct glycan coupled to glass surfaces as a model of sperm interaction with the oviduct Article Snippet: Two functionalized glass surfaces were used to immobilize oviduct glycans and model the oviduct reservoir (Fig. ): |
