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streptavidin coverslips  (PolyAn GmbH)


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    Structured Review

    PolyAn GmbH streptavidin coverslips
    Schematic representation of the two in vitro systems developed to model the sperm-oviduct interaction. ( a ) 3-D functionalized <t>streptavidin</t> coverslip: the <t>coverslips</t> are placed on a petri dish, medium droplets containing sulfated Le X (suLe X ) are placed on the streptavidin regions of the coverslip that are surrounded by hydrophobic surface, and the whole system is covered with mineral oil. ( b ) Polyethyleneglycol (PEG)-biotin slides: the slides are coated with streptavidin, a hybridization chamber is placed on the slide, and the chambers are filled with medium containing suLe X . ( c ) The streptavidin coating in both glass surfaces allows biotinylated suLe X to be tightly immobilized to the surface. Once the sperm are added and incubated in the system, membrane receptors on the head recognize and attach to suLe X bound to the glass.
    Streptavidin Coverslips, supplied by PolyAn GmbH, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/streptavidin+coverslips/Glass+coverslips+covalent+Streptavidin+surface/pmc11806115-199-18-20
    Average 93 stars, based on 1 article reviews
    streptavidin coverslips - by Bioz Stars, 2026-10
    93/100 stars

    Images

    1) Product Images from "Porcine sperm bind to an oviduct glycan coupled to glass surfaces as a model of sperm interaction with the oviduct"

    Article Title: Porcine sperm bind to an oviduct glycan coupled to glass surfaces as a model of sperm interaction with the oviduct

    Journal: Scientific Reports

    doi: 10.1038/s41598-025-88986-2

    Schematic representation of the two in vitro systems developed to model the sperm-oviduct interaction. ( a ) 3-D functionalized streptavidin coverslip: the coverslips are placed on a petri dish, medium droplets containing sulfated Le X (suLe X ) are placed on the streptavidin regions of the coverslip that are surrounded by hydrophobic surface, and the whole system is covered with mineral oil. ( b ) Polyethyleneglycol (PEG)-biotin slides: the slides are coated with streptavidin, a hybridization chamber is placed on the slide, and the chambers are filled with medium containing suLe X . ( c ) The streptavidin coating in both glass surfaces allows biotinylated suLe X to be tightly immobilized to the surface. Once the sperm are added and incubated in the system, membrane receptors on the head recognize and attach to suLe X bound to the glass.
    Figure Legend Snippet: Schematic representation of the two in vitro systems developed to model the sperm-oviduct interaction. ( a ) 3-D functionalized streptavidin coverslip: the coverslips are placed on a petri dish, medium droplets containing sulfated Le X (suLe X ) are placed on the streptavidin regions of the coverslip that are surrounded by hydrophobic surface, and the whole system is covered with mineral oil. ( b ) Polyethyleneglycol (PEG)-biotin slides: the slides are coated with streptavidin, a hybridization chamber is placed on the slide, and the chambers are filled with medium containing suLe X . ( c ) The streptavidin coating in both glass surfaces allows biotinylated suLe X to be tightly immobilized to the surface. Once the sperm are added and incubated in the system, membrane receptors on the head recognize and attach to suLe X bound to the glass.

    Techniques Used: In Vitro, Hybridization, Incubation, Membrane

    Schematic representation of IVF using sperm bound to glycans pre-fertilization (glycan-IVF). Functionalized streptavidin-coverslips were coated with suLe X in droplets of medium. Sperm were added to the droplets and incubated to allow them to bind suLe X . The medium in the droplet was replaced to remove free sperm. Mature cumulus-oocyte complexes (COCs) were added to the droplet and co-cultured with bound sperm. COCs induce the release of some of the sperm enabling them to fertilize the oocytes.
    Figure Legend Snippet: Schematic representation of IVF using sperm bound to glycans pre-fertilization (glycan-IVF). Functionalized streptavidin-coverslips were coated with suLe X in droplets of medium. Sperm were added to the droplets and incubated to allow them to bind suLe X . The medium in the droplet was replaced to remove free sperm. Mature cumulus-oocyte complexes (COCs) were added to the droplet and co-cultured with bound sperm. COCs induce the release of some of the sperm enabling them to fertilize the oocytes.

    Techniques Used: Incubation, Cell Culture

    Related Articles

    In Vitro:

    Article Title: Porcine sperm bind to an oviduct glycan coupled to glass surfaces as a model of sperm interaction with the oviduct.
    Article Snippet: Two functionalized glass surfaces were used to immobilize oviduct glycans and model the oviduct reservoir (Fig. 1): 3D-functionalized streptavidin coverslips (PolyAn Scientific, Berlin, Germany) and functionalized PEG-biotin slides (Microsurfaces Inc., Englewood, NJ).

    Article Title: Porcine sperm bind to an oviduct glycan coupled to glass surfaces as a model of sperm interaction with the oviduct
    Article Snippet: Two functionalized glass surfaces were used to immobilize oviduct glycans and model the oviduct reservoir (Fig. ): 3D-functionalized streptavidin coverslips (PolyAn Scientific, Berlin, Germany) and functionalized PEG-biotin slides (Microsurfaces Inc., Englewood, NJ).

    Hybridization:

    Article Title: Porcine sperm bind to an oviduct glycan coupled to glass surfaces as a model of sperm interaction with the oviduct.
    Article Snippet: Two functionalized glass surfaces were used to immobilize oviduct glycans and model the oviduct reservoir (Fig. 1): 3D-functionalized streptavidin coverslips (PolyAn Scientific, Berlin, Germany) and functionalized PEG-biotin slides (Microsurfaces Inc., Englewood, NJ).

    Article Title: Porcine sperm bind to an oviduct glycan coupled to glass surfaces as a model of sperm interaction with the oviduct
    Article Snippet: Two functionalized glass surfaces were used to immobilize oviduct glycans and model the oviduct reservoir (Fig. ): 3D-functionalized streptavidin coverslips (PolyAn Scientific, Berlin, Germany) and functionalized PEG-biotin slides (Microsurfaces Inc., Englewood, NJ).

    Incubation:

    Article Title: Porcine sperm bind to an oviduct glycan coupled to glass surfaces as a model of sperm interaction with the oviduct.
    Article Snippet: Two functionalized glass surfaces were used to immobilize oviduct glycans and model the oviduct reservoir (Fig. 1): 3D-functionalized streptavidin coverslips (PolyAn Scientific, Berlin, Germany) and functionalized PEG-biotin slides (Microsurfaces Inc., Englewood, NJ).

    Article Title: Porcine sperm bind to an oviduct glycan coupled to glass surfaces as a model of sperm interaction with the oviduct
    Article Snippet: Two functionalized glass surfaces were used to immobilize oviduct glycans and model the oviduct reservoir (Fig. ): 3D-functionalized streptavidin coverslips (PolyAn Scientific, Berlin, Germany) and functionalized PEG-biotin slides (Microsurfaces Inc., Englewood, NJ).

    Membrane:

    Article Title: Porcine sperm bind to an oviduct glycan coupled to glass surfaces as a model of sperm interaction with the oviduct.
    Article Snippet: Two functionalized glass surfaces were used to immobilize oviduct glycans and model the oviduct reservoir (Fig. 1): 3D-functionalized streptavidin coverslips (PolyAn Scientific, Berlin, Germany) and functionalized PEG-biotin slides (Microsurfaces Inc., Englewood, NJ).

    Article Title: Porcine sperm bind to an oviduct glycan coupled to glass surfaces as a model of sperm interaction with the oviduct
    Article Snippet: Two functionalized glass surfaces were used to immobilize oviduct glycans and model the oviduct reservoir (Fig. ): 3D-functionalized streptavidin coverslips (PolyAn Scientific, Berlin, Germany) and functionalized PEG-biotin slides (Microsurfaces Inc., Englewood, NJ).

    Cell Culture:

    Article Title: Porcine sperm bind to an oviduct glycan coupled to glass surfaces as a model of sperm interaction with the oviduct.
    Article Snippet: Two functionalized glass surfaces were used to immobilize oviduct glycans and model the oviduct reservoir (Fig. 1): 3D-functionalized streptavidin coverslips (PolyAn Scientific, Berlin, Germany) and functionalized PEG-biotin slides (Microsurfaces Inc., Englewood, NJ).

    Article Title: Porcine sperm bind to an oviduct glycan coupled to glass surfaces as a model of sperm interaction with the oviduct
    Article Snippet: Two functionalized glass surfaces were used to immobilize oviduct glycans and model the oviduct reservoir (Fig. ): 3D-functionalized streptavidin coverslips (PolyAn Scientific, Berlin, Germany) and functionalized PEG-biotin slides (Microsurfaces Inc., Englewood, NJ).



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    Schematic representation of the two in vitro systems developed to model the sperm-oviduct interaction. ( a ) 3-D functionalized <t>streptavidin</t> coverslip: the <t>coverslips</t> are placed on a petri dish, medium droplets containing sulfated Le X (suLe X ) are placed on the streptavidin regions of the coverslip that are surrounded by hydrophobic surface, and the whole system is covered with mineral oil. ( b ) Polyethyleneglycol (PEG)-biotin slides: the slides are coated with streptavidin, a hybridization chamber is placed on the slide, and the chambers are filled with medium containing suLe X . ( c ) The streptavidin coating in both glass surfaces allows biotinylated suLe X to be tightly immobilized to the surface. Once the sperm are added and incubated in the system, membrane receptors on the head recognize and attach to suLe X bound to the glass.
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    Image Search Results


    Schematic representation of the two in vitro systems developed to model the sperm-oviduct interaction. ( a ) 3-D functionalized streptavidin coverslip: the coverslips are placed on a petri dish, medium droplets containing sulfated Le X (suLe X ) are placed on the streptavidin regions of the coverslip that are surrounded by hydrophobic surface, and the whole system is covered with mineral oil. ( b ) Polyethyleneglycol (PEG)-biotin slides: the slides are coated with streptavidin, a hybridization chamber is placed on the slide, and the chambers are filled with medium containing suLe X . ( c ) The streptavidin coating in both glass surfaces allows biotinylated suLe X to be tightly immobilized to the surface. Once the sperm are added and incubated in the system, membrane receptors on the head recognize and attach to suLe X bound to the glass.

    Journal: Scientific Reports

    Article Title: Porcine sperm bind to an oviduct glycan coupled to glass surfaces as a model of sperm interaction with the oviduct

    doi: 10.1038/s41598-025-88986-2

    Figure Lengend Snippet: Schematic representation of the two in vitro systems developed to model the sperm-oviduct interaction. ( a ) 3-D functionalized streptavidin coverslip: the coverslips are placed on a petri dish, medium droplets containing sulfated Le X (suLe X ) are placed on the streptavidin regions of the coverslip that are surrounded by hydrophobic surface, and the whole system is covered with mineral oil. ( b ) Polyethyleneglycol (PEG)-biotin slides: the slides are coated with streptavidin, a hybridization chamber is placed on the slide, and the chambers are filled with medium containing suLe X . ( c ) The streptavidin coating in both glass surfaces allows biotinylated suLe X to be tightly immobilized to the surface. Once the sperm are added and incubated in the system, membrane receptors on the head recognize and attach to suLe X bound to the glass.

    Article Snippet: Two functionalized glass surfaces were used to immobilize oviduct glycans and model the oviduct reservoir (Fig. ): 3D-functionalized streptavidin coverslips (PolyAn Scientific, Berlin, Germany) and functionalized PEG-biotin slides (Microsurfaces Inc., Englewood, NJ).

    Techniques: In Vitro, Hybridization, Incubation, Membrane

    Schematic representation of IVF using sperm bound to glycans pre-fertilization (glycan-IVF). Functionalized streptavidin-coverslips were coated with suLe X in droplets of medium. Sperm were added to the droplets and incubated to allow them to bind suLe X . The medium in the droplet was replaced to remove free sperm. Mature cumulus-oocyte complexes (COCs) were added to the droplet and co-cultured with bound sperm. COCs induce the release of some of the sperm enabling them to fertilize the oocytes.

    Journal: Scientific Reports

    Article Title: Porcine sperm bind to an oviduct glycan coupled to glass surfaces as a model of sperm interaction with the oviduct

    doi: 10.1038/s41598-025-88986-2

    Figure Lengend Snippet: Schematic representation of IVF using sperm bound to glycans pre-fertilization (glycan-IVF). Functionalized streptavidin-coverslips were coated with suLe X in droplets of medium. Sperm were added to the droplets and incubated to allow them to bind suLe X . The medium in the droplet was replaced to remove free sperm. Mature cumulus-oocyte complexes (COCs) were added to the droplet and co-cultured with bound sperm. COCs induce the release of some of the sperm enabling them to fertilize the oocytes.

    Article Snippet: Two functionalized glass surfaces were used to immobilize oviduct glycans and model the oviduct reservoir (Fig. ): 3D-functionalized streptavidin coverslips (PolyAn Scientific, Berlin, Germany) and functionalized PEG-biotin slides (Microsurfaces Inc., Englewood, NJ).

    Techniques: Incubation, Cell Culture

    KEY RESOURCES TABLE

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    Article Title: Microglia degrade Alzheimer’s amyloid-beta deposits extracellularly via digestive exophagy

    doi: 10.1016/j.celrep.2024.115052

    Figure Lengend Snippet: KEY RESOURCES TABLE

    Article Snippet: Streptavidin-coated coverslips , Nanocs , CS-SV-5.

    Techniques: Recombinant, Cell Culture, Electron Microscopy, Software, Polymer, Microscopy, Spectrophotometry, Membrane, Transmission Assay